Part:BBa_K3956008:Experience

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Applications of BBa_K3956008

This protein domain was inserted in an enzyme cut/bridging sequence and a PRM9 terminator found from a study by Curran et. al, in CCA_San_Diego's 2021 project to make synthetic collagen.

Although we were able to verify that the construct had been correctly inserted into appropriate selective plates by colony PCR verification, the protein expression was not able to be verified within the time constraints of the competition. We want to emphasize that this sequence may still be successful, but its efficiency was unable to be demonstrated within the time span that CCA_San_Diego had in the lab. For future teams, we advise testing construct designs with successful expression with and without the sequence to compare expression levels.

User Reviews

UNIQb8bbe10deb8bcdbe-partinfo-00000000-QINU UNIQb8bbe10deb8bcdbe-partinfo-00000001-QINU